Xo4 citing papers

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2020

Structures of cell wall arabinosyltransferases with the anti-tuberculosis drug ethambutol Lu Zhang, Yao Zhao, Yan Gao, Lijie Wu, Ruogu Gao, Qi Zhang, Yinan Wang, Chengyao Wu, Fangyu Wu, Sudagar S. Gurcha, Natacha Veerapen, Sarah M. Batt, Wei Zhao, Ling Qin, Xiuna Yang, Manfu Wang, Yan Zhu, Bing Zhang, Lijun Bi, Xian’en Zhang, Haitao Yang, Luke W. Guddat, Wenqing Xu, Quan Wang, Jun Li, Gurdyal S. Besra, Zihe Rao (2020) – Science  23 Apr 2020: eaba9102 DOI: 10.1126/science.aba9102

Crystal structure of Mokola virus glycoprotein in its post-fusion conformation Belot L, Ouldali M, Roche S, Legrand P, Gaudin Y, et al. (2020) – PLOS Pathogens 16(3): e1008383. https://doi.org/10.1371/journal.ppat.1008383

The C‐terminal domain of Corynebacterium glutamicum mycoloyltransferase A is composed of five repeated motifs involved in cell wall binding and stability C Dietrich, I Li de la Sierra‐Gallay, M Masi, E Girard, N Dautin, F Constantinesco‐Becker, M Tropis, M Daffé, H van Tilbeurgh, N Bayan – Molecular Microbiology, published February 2020 https://doi.org/10.1107/s2053230x1901642x

The adhesive PitA pilus protein from the early dental plaque colonizer Streptococcus oralis: expression, purification, crystallization and X-ray diffraction analysis Rajnesh Kumari Yadav, Vengadesan Krishnan – Acta Crystallographica Section F: Structural Biology Communications, 76(Pt 1), 8-13, January 2020 https://doi.org/10.1107/s2053230x1901642x

2019

Molecular insight into a new low‐affinity xylan binding module from the xylanolytic gut symbiont Roseburia intestinalis Maria Louise Leth, Morten Ejby, Eva Madland, Yoshihito Kitaoku, Dirk J. Slotboom, Albert Guskov, Finn Lillelund Aachmann, Maher Abou Hachem – The FEBS Journal, November 2019 https://doi.org/10.1111/febs.15117

Crystal structures of the Bacillus subtilis prophage lytic cassette proteins XepA and YomS Stefanie Freitag-Pohl, Andrius Jasilionis, Maria Håkansson, L. Anders Svensson, Rebeka Kovačič, Martin Welin, Hildegard Watzlawick, Lei Wang, Josef Altenbuchner, Magdalena Płotka, Anna Karina Kaczorowska, Tadeusz Kaczorowski, Eva Nordberg Karlsson, Salam Al-Karadaghi, Björn Walse, Arnthór Aevarsson, Ehmke Pohl – Acta Crystallographica Section D, Structural Biology, 75(11), 1028-1039, November 2019 https://doi.org/10.1107/s2059798319013330

Marine Proteobacteria metabolize glycolate via the β-hydroxyaspartate cycle Lennart Schada von Borzyskowski, Francesca Severi, Karen Krüger, Lucas Hermann, Alexandre Gilardet, Felix Sippel, Bianca Pommerenke, Peter Claus, Niña Socorro Cortina, Timo Glatter, Stefan Zauner, Jan Zarzycki, Bernhard M. Fuchs, Erhard Bremer, Uwe G. Maier, Rudolf I. Amann, Tobias J. Erb – Nature, 575(7783), 500-504, November 2019 https://doi.org/10.1038/s41586-019-1748-4

The archaeal LDH-like malate dehydrogenase from Ignicoccus islandicus displays dual substrate recognition, hidden allostery and a non-canonical tetrameric oligomeric organization Jennifer Roche, Eric Girard, Caroline Mas, Dominique Madern – Journal of Structural Biology, 208(1), 7-17, October 2019 https://doi.org/10.1016/j.jsb.2019.07.006

A simple and versatile microfluidic device for efficient biomacromolecule crystallization and structural analysis by serial crystallographe Raphaël de Wijn, Oliver Hennig, Jennifer Roche, Sylvain Engilberge, Kevin Rollet, Pablo Fernandez-Millan, Karl Brillet, Heike Betat, Mario Mörl, Alain Roussel, Eric Girard, Christoph Mueller-Dieckmann, Gavin C. Fox, Vincent Olieric, José A. Gavira, Bernard Lorber, Claude Sauter – IUCrJ, 6(Pt 3), 454-464, May 2019 https://doi.org/10.1107/s2052252519003622

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Various additives were tested to improve crystal quality and size.  The best results were achieved with the recently described nucleating and phasing agent Tb-Xo4.

Nature  575, 500–504 (2019).

https://doi.org/10.1038/s41586-019-1748-4
Nature
Schada von Borzyskowski & al.Marine Proteobacteria metabolize glycolate via the β-hydroxyaspartate cycle.

 

Beauty is in the eye of the beholder! - These sorta ugly Tb (Xo4) co-crystals enabled us to solve a tricky phase problem (originally P1 space group, indifferent resolution, yada yada) which has been plaguing us for almost 5 years

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