Xo4/Crystallophores are straightforward Experimental Phasing Agents facilitating crystal structure determination using SAD or MAD methods, due to the exceptional anomalous properties of f-block elements. They overcome the tedious and time-consuming work of seleno-methionine labelling or of heavy-atom incorporation.
Xo4 are fully compatible with conventional phasing methods. Prior to data collection, carry out a rapid (from 30 sec to 5 min) soak of crystals in a concentrated Xo4 solution (100mM) in mother liquor supplemented with a cryo-protectant.
Moreover, at the request of NatX-Ray, our range of lanthanide complexes manufactured by Polyvalan has just been completed by 4 products complementary to our Crystallophore phasing agents.
AVOID BACK SOAKING